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gabR [2017-06-21 20:16:46]
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gabR [2017-06-21 20:16:46]

transcription activator of gabT-gabD, repressor of gabR (MocR/ GabR family)
locus
BSU03890
pI
6.71
mw
55.00 kDa
protein length
479 aa Sequence Blast
gene length
1437 bp Sequence Blast
function
regulation of gamma-amino butyric acid utilization
product
transcription regulator
essential
no
synonyms
ycnF
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.2|Utilization of amino acids] → [category|SW 2.3.2.10|Utilization of gamma-amino butyric acid]
  • [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.2|Transcription factors and their control] → [category|SW 3.4.2.5|Transcription factors/ other]

    • Gene

    Coordinates
    440,025 → 441,464

    The protein

    Catalyzed reaction/ biological activity

  • transcription activation of the ''[gene|4E1FD02C6FE4103E3B4942BF5221C062CB8D0B9B|gabT]-[gene|A0BEB92D54799956A4ADE106A1388E5710141069|gabD]'' operon in the presence of GABA and PLP, transcription repression of ''[gene|C7C36FAC0CE72226960FC7E8E016B8EC77AF1036|gabR]'' [Pubmed|15223311]

    • Protein family

  • [[MocR/ GabR family]] [Pubmed|22020104]

    • Paralogous protein(s)

  • none, but there are 7 members of the [[MocR/ GabR family]] in ''B. subtilis

    • [SW|Domains]

  • N-terminal DNA-binding helix-turn-helix motif (corresponding to domains of the [SW|GntR family]) [Pubmed|24127574]
  • C-terminal domain is homologous to PLP-binding large domain of aminotransferases [Pubmed|24127574]

    • [SW|Cofactors]

  • gamma-aminobutyric acid, PLP [Pubmed|24127574]

    • Effectors of protein activity

  • activation of ''[gene|4E1FD02C6FE4103E3B4942BF5221C062CB8D0B9B|gabT]-[gene|A0BEB92D54799956A4ADE106A1388E5710141069|gabD]'' expression is triggered by gamma-amino butyrate and pyridoxalphosphate [Pubmed|15223311]

    • Structure

  • [PDB|4MGR] (the apo-protein) [Pubmed|24127574]
  • [PDB|4N0B] (the complex with PLP) [Pubmed|24127574]

    • Expression and Regulation

    Operons

    genes
    [gene|C7C36FAC0CE72226960FC7E8E016B8EC77AF1036|gabR]
    description
    [pubmed|22383849]

    sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|12123465], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

    • regulatory mechanism

  • [protein|C7C36FAC0CE72226960FC7E8E016B8EC77AF1036|GabR]: negative autoregulation, [Pubmed|12123465], in [regulon|C7C36FAC0CE72226960FC7E8E016B8EC77AF1036|GabR regulon]
    • view in new tab

    Biological materials

    Mutant

  • MGNA-C014 (ycnF::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2012 NBRP B. subtilis, Japan]

    • Labs working on this gene/protein

  • [SW|Linc Sonenshein], Tufts University, Boston, MA, USA [http://www.tufts.edu/sackler/microbiology/faculty/sonenshein/index.html Homepage]

    • References

  • 11756427,15223311,12123465,24127574,22020104,25388514,25911692,27640111,26681693,28348215,28412355